Determination of the substitution pattern of cationic starch ethers

摘要

Degradation of cationic starches and subsequent permethylation and Hofmann-elimination of the methyl-glucosides yields the corresponding enolether derivatives, which can be analyzed by GLC and GLC-MS. The relative ratio of 2-, 3- and 6-substitution in the anhydroglucose unit as well as of un-, mono-, di- and trisubstitution in the anhydroglucose unit as well as of un-, mono-, di-and trisubstituted monomers can be determined. The experimental results are compared with the data calculated by means of a kinetic model. Only slight deviations from a random distribution in the anhydroglucose unit are observed and discussed. Enzymatic degradation of the cationic starch ethers and measurement of the liberated glucose should give indication to the distribution along the polymer chain.