Catalytic spectrofluorimetric determination of superoxide anion radical and superoxide dismutase activity using N,N-dimethylaniline as the substrate for horseradish peroxidase (HRP)

摘要

The coupled reaction of N,N-dimethylaniline (DMA) with 4-aminoantipyrine (4-AAP) using superoxide anion radical (02) as oxidizing agent under the catalysis of horseradish peroxidase (HRP) was studied. Based on the reaction, O-2(-) produced by irradiating Vitamin B-2, (V-B2) was spectrophotometricly determined at 554 nm. The linear range of this method was 1.8 X 10(-6) - 1.2 x 10(-4) mol l(-1) with a detection limit of 5.3 x 10(-7) mol l(-1). The effect of interferences on the determination of O-2(-) was investigated. The proposed method was successfully applied to the determination of superoxide dismutase (SOD) activity in human blood and mouse blood. (C) 2002 Elsevier Science B.V. All rights reserved. [References: 10]