Expressed sequence tags and new gene coding regions from the horn fly

摘要

We have used the expressed sequence tag (EST) approach to isolate new gene coding regions from the horn fly, Haematobia irritans. Seventy four unique ESTs and one complete open reading frame were sequenced from genes not previously identified in horn flies and recorded in the databases of the National Center for Biotechnology Information. Fifteen ESTs are from genes involved in metabolism, ten ESTs are from structural proteins, and 20 ESTs are from genes involved in other functions, including storage, heat shock response and transcription. Five of the ESTs have similarity to members of gene families involved in resistance to pesticides. The entire coding region from a gene involved in cell cycle regulation was obtained and the mRNA encoding the protein found to be highly expressed in early embryonic and pupal stages of the fly.