Efficacy of certain fungicides and bio-control agents in control of post-harvest decay of mango fruits due to Colletotrichum gloeosporiodes in vitro and in vivo.

摘要

A study was conducted to evaluate the efficacy of some fungicides (carbendazim, benomyl and thiophanate-methyl) and biological control agents (Trichoderma harzianum and Bacillus subtilis) in controlling anthracnose (Colletotrichum gloeosporioides [Glomerella cingulata]) infecting mango cv. Baneshan fruits in vitro and in vivo. In the in vitro studies, the fungicides were applied at 250, 500 and 1000 ppm. In the in vivo studies, the fungicides were applied at 0.1%, while T. harzianum and B. subtilis were applied at 106 spores/ml and 108 cells/ml, respectively. The disease index was calculated using the 0-10 scale. In in vitro studies, benomyl at 1000 ppm gave 80 and 70% mycelial growth inhibition at 6 and 8 days after inoculation (DAI), respectively, and proved to be significantly superior over the other treatments. This was followed by carbendazim at 1000 ppm. Both biological control agents significantly reduced the growth of C. gloeosporioides; however, the reduction was relatively higher with T. harzianum (61.25 and 55.71% at 6 and 8 DAI, respectively) than with B. subtilis (45.00 and 38.57%). In in vivo studies, benomyl at 0.1% was highly effective in controlling anthracnose with 93.59 and 90.12% disease control and 1.00 and 1.33 disease index at 4 and 8 DAI, respectively. T. harzianum resulted in the better control of anthracnose (60.73 and 60.52%) than B. subtilis (54.75%) at 4 and 8 DAI..