Application of Gradient Elution Optimized by ChromSword Software in Chromatography of Phenothiazines in Reversed Phase Systems Controlled by Chaotropic Effect

摘要

In this study, gradient and isocratic elution of 11 phenothiazine derivatives in the reversed--phase systems controlled by chaotropic effect was compared. Potential applicability of the Chromsword software for retention data prediction either under isocratic or gradient conditions was studied. Simulations were performed on the basis of previously conducted experiments concerning retention measurements at different concentrations of organic modifiers (methanol, acetonitrile) in the mobile phase. Eluent systems were acidified with perchloric acid and modified with chaotropic additives (sodium perchlorate and sodium hexafluorophosphate) at the constant concentration. An agreement between the predicted and experimental retention parameters was estimated statistically. Optimal gradient profile for the separation of all examined compounds was selected.