Molecular characterisation of the Xenopus laevis chaperonin gene Cctg

摘要

By library screening and FCR we have obtained cDNA clones which encode the gamma subunil of the CCT chaperonin complex from Xenopus laei'is. The gene (XlCctg), which encodes the CCT 7 subunil contains an open reading frame which codes for 547 aniino acid residues (60 kDa) anil the predicted aniino acid sequence shares a high degree of sequence identity with other CCVy homologues. The XlCrtu mRNA measures 2.1 kb and is expressed ubiquitously in till of the X. laevis tissues examined. The mRNA levels of XlCctn are significantly higher in the ovary compared vvitli other tissues.