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Vitrification as a prospect for cryopreservation of tissue-engineered constructs

机译:玻璃化作为组织工程构建体冷冻保存的前景

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Cryopreservation plays a significant function in tissue banking and will presume yet larger value when more and more tissue-engineered products will routinely enter the clinical arena. The most common concept underlying tissue engineering is to combine a scaffold (cellular solids) or matrix (hydrogels) with living cells to form a tissue-engineered construct (TEC) to promote the repair and regeneration of tissues. The scaffold and matrix are expected to support cell colonization, migration, growth and differentiation, and to guide the development of the required tissue. The promises of tissue engineering, however, depend on the ability to physically distribute the products to patients in need. For this reason, the ability to cryogenically preserve not only cells, but also TECs, and one day even whole laboratory-produced organs, may be indispensable. Cryopreservation can be achieved by conventional freezing and vitrification (ice-free cryopreservation). In this publication we try to define the needs versus the desires of vitrifying TECs, with particular emphasis on the cryoprotectant properties, suitable materials and morphology. It is concluded that the formation of ice, through both direct and indirect effects, is probably fundamental to these difficulties, and this is why vitrification seems to be the most promising modality of cryopreservation. (c) 2006 Elsevier Ltd. All rights reserved.
机译:冷冻保存在组织储备中起着重要作用,并且当越来越多的组织工程产品常规进入临床领域时,冷冻保存将具有更大的价值。组织工程中最常见的概念是将支架(细胞固体)或基质(水凝胶)与活细胞结合在一起,以形成组织工程构建体(TEC),从而促进组织的修复和再生。支架和基质有望支持细胞定植,迁移,生长和分化,并指导所需组织的发育。但是,组织工程学的前景取决于将产品物理分配给需要的患者的能力。因此,不仅需要低温保存细胞,而且还需要保存TECs的能力,甚至有一天甚至可以保存整个实验室生产的器官。冷冻保存可以通过常规的冷冻和玻璃化(无冰冷冻保存)来实现。在本出版物中,我们尝试定义玻璃化TEC的需求与需求,特别强调防冻性能,合适的材料和形态。结论是,通过直接和间接作用形成冰可能是这些困难的根本原因,这就是为什么玻璃化似乎是最有希望的低温保存方式的原因。 (c)2006 Elsevier Ltd.保留所有权利。

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