Effect of Exogenous Factors on the Induction of Spicule Formation in Sea Urchin Embryonic Cell Cultures

摘要

The effect of exogenous factors on the realization of the spicule formation program in two sea urchin species, Strongylocentrotus intermedius and S. nudus, has been studied in primary embryonic cell cultures derived from the blastula and gastrula stages. It has been shown that the process of spicule formation depends on the type of substrate and the composition of the medium. An original finding is that calf or horse serum necessary for spicule formation in vitro can be replaced by a complex of factors including insulin, transferrin, and lectins. Methods allowing control over the growth and differentiation of marine invertebrate embryonic cells in vitro open prospects for their application to practical problems such as the establishment of cell cultures producing certain mineral structures.