Cells of Mouse Submandibular Salivary Gland in Culture in vitro

摘要

A cell culture that preserves its phenotype up to the 20th passage was obtained from mouse submandibular salivary glands. An analysis of the heterogeneous culture indicates the existence of several morphological types of cells, including small, densely packed cells of cuboidal or polygonal shapes and large, rounded cells. Epithelial cells of the submandibular gland cultured for several weeks were able to form tubular structures. Our studied cell culture of glandulocytes (cells of glandular epithelium) was represented by K19-and NGF-positive cells. It is important to note that, using both immunocytochemical staining and PCR, the expression of genes that encode the proinsulin and insulin proteins is revealed in the studied cell population.