Low-frequency electromagnetic field exposure accelerates chondrocytic phenotype expression on chitosan substrate.

摘要

To find whether low-frequency pulsed electromagnetic fields help repair larger cartilage defects with the assistance of tissue-engineered scaffolds, we tested their effect on the behavior of chondrocyte cells cultured on chitosan films. Primary porcine chondrocytes growing on chitosan films were exposed to low-frequency pulsed electromagnetic fields (frequency=75 Hz; impulse width=1.3 ms; strength=1.8-3 mT) 2 hours a day for 3 weeks. The cells that were not exposed to low-frequency pulsed electromagnetic fields served as controls. For 3 weeks, cell proliferation, viability, and expressions of type II collagen and glycosaminoglycan were measured weekly. Cell morphology and histological stains of glycosaminoglycan and type II collagen were performed at the end of the test. The cell proliferation and viability of the low-frequency pulsed electromagnetic fields group and the control were similar each week. By the end of the third week, cells in the low-frequency pulsed electromagnetic fields group deposited 28% more glycosaminoglycan than the control cells. The amounts of type II collagen deposited in the low-frequency pulsed electromagnetic fields group were 24% and 27% higher than those of the control group by week 2 and 3, respectively. Histological and immunohistochemical analyses confirmed the releases of glycosaminoglycan and type II collagen. Cells from both groups grew in aggregates and possessed a spherical shape after 3 weeks. These results suggest that low-frequency pulsed electromagnetic fields can enhance extracellular matrix production on chitosan substrate. Combining tissue engineering and low-frequency pulsed electromagnetic fields could further accelerate cartilage repair.