Pathological and PCR profile of a high mortality canary poxvirus (CAPV) outbreak.

摘要

Canary poxyvirus (CAPV) is a highly infectious disease which may result in 100% mortality. Pathological and molecular findings taken from 200 colored-breeder canaries affected by CAPV with a mortality rate of 98.5% are reported. Prior to death, severeconjunctivitis, dyspnoea, anorexia, weakness, rapid loss of weight, fluffed-up appearance and cutaneous proliferative lesions around the eyes and the beak occurred. At necropsy cutaneous lesions around the eyes and/or base of the beak were characterizedby epidermal hypertrophy and hyperplasia with degeneration of the stratified squamous epithelium. Intracytoplasmic inclusions in hypertrophied epithelial cells with typical Bollinger bodies occurred. Edema, hyperemia and hemorrhages in the lungs and fibrinous pneumonitis was observed. PCR results revealed 578 base pairs (bp) pox virus DNA present in eyelids, skin and lung. However, PCR did not reveal pox virus DNA in liver or cardiac tissues.