Development and validation of RP-HPLC method for the estimation of lovastatin in bulk and tablet dosage form

摘要

A simple, rapid, sensitive and precise High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of lovastatin in pure and tablet dosage form. In this method RP-C_(18) column (250mmx4.6mm I.D., 5μm particle size) with mobile phase consisting of acetonitrile, methanol and water in the ratio of 79:12:9 v/v/v in isocratic mode was used. The detection wavelength is 230nm and the flow rate 1.2ml/min.The linearity was found in the range of 10-100μg/ml and shows a correlation coefficient of 0.9997. The proposed method was validated by determining sensitivity, accuracy, precision and linearity. The proposed method is simple, fast, accurate, precise and reproducible hence can be applied for routine quality control analysis of lovastatin in pure and tablet dosage form.