The association of IL-6 and IL-6R gene polymorphisms with chronic periodontitis in a Chinese population

摘要

Objective: The aim of our investigation is to reveal the association of interleukin-6 (IL-6) and interleukin-6 receptor (IL-6R) gene polymorphisms with chronic periodontitis in Northwestern Chinese Han Population. Material and Methods: A case-control study of 199 cases of chronic periodontitis patients and 216 healthy controls was performed. Genomic DNA was isolated from whole venous blood, and four single nucleotide polymorphisms (IL-6 -572 G/C and -1363 G/T, IL-6R -183 G/A and +48892 A/C) were analysed using polymerase chain reaction, followed by restriction fragment length polymorphism analysis and sequencing methods. Results: Our SNP analyses showed that the distribution of the IL-6 -572 G/C and IL-6R -183 G/A polymorphisms did not differ between patients and controls. The frequency of the IL-6 -1363 G/T genotype GG was significantly increased chronic periodontitis cases (P = 0.023, odds ratio (OR) = 2.825 adjusted for gender and age.) The IL-6R +48892 A/C polymorphisms genotype CC was found to be protective against chronic periodontitis (P = 0.004, OR = 0.318 adjusted for gender and age.). In addition, compared with the other haplotypes, haplotype A+48892A-183 was significantly associated with chronic periodontitis, with an odds ratio of 0.720 (P = 0.0235). Conclusion: Our results suggest that the IL-6 -1363 G/T and IL-6R +48892 A/C polymorphisms may contribute to genetic susceptibility to chronic periodontitis in Northwestern Chinese Han population.