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In Vitro Prevention of Browning in Plantain Culture

机译:车前草中褐变的体外预防

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The in vitro propagation of plantain (Musa paradisiaca) is still faced with lots of challenges such as blackening or browning of tissues prior to culture due to the oxidation of phenolic compound by polyphenolic oxidase enzyme present in the tissue when excised. Approach: Understanding browning processes in plantain and possible ways of minimizing it during excision of explants with particular emphasis on the use of antioxidants was purposed. Tissues were surfaced sterilized with 0.1% (w/v) HgCL_2 for 1-6 min to get a pure culture and then treated for 2 h with different concentrations of 0.1-0.5 mg mL~(-1) of potassium citrate and citrate (K-C: C) as an antioxidant to check browning while sterile distilled water was used as control. Results and Conclusion: The result showed that contamination free culture (100%) was achieved in the explants treated with HgCL_2 for 6 min. Also the various concentration of K-C: C prevented browning within 2 h before culturing the tissues inferring that browning in young plantain excised tissue can be greatly reduced by presoaking or pretreatment with antioxidant solution of potassium citrate-citrate before culturing them.
机译:车前草(Musa paradisiaca)的体外繁殖仍然面临许多挑战,例如在培养前组织变黑或褐变,这是由于切除后组织中存在的多酚氧化酶会氧化酚类化合物。方法:旨在了解车前草中的褐变过程以及在外植体切除过程中将其最小化的可能方法,特别强调使用抗氧化剂。用0.1%(w / v)HgCL_2对组织进行表面灭菌1-6分钟以得到纯培养物,然后用0.1-0.5 mg mL〜(-1)柠檬酸钾和柠檬酸钾(KC)的不同浓度处理2小时:C)作为抗氧化剂以检查褐变,同时使用无菌蒸馏水作为对照。结果与结论:结果表明,用HgCL_2处理6分钟的外植体实现了无污染的培养(100%)。同样,各种浓度的K-C:C可以防止在培养组织前2 h内褐变,这表明在培养前,用柠檬酸柠檬酸钾的抗氧化溶液进行预浸泡或预处理可以大大减少年轻大蕉切除组织中的褐变。

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