Preparation of Kudzu vine oral formulation and its application against doxorubicin-induced cardiotoxicity via alteration of gut microbiota

摘要

In this experimental protocol,we scrutinized the preventive effect of Kudzu vine extract on DOX-induced cardiac toxicity via alteration of gut microbiota.Sprague-Dawley(SD)was used in this protocol and the rats were divided into different groups and received the DOX(3 mg/kg)for induction the cardiac remodeling and Kudzu vine extract(50,100 and 200 mg/kg)administration was used for the estimation of cardioprotective potential for 5 weeks.Afterward,biochemical parameter,electrolyte,antioxidant,cytokines and inflammatory parameters were estimated.Kudzu vine remarkably improved the body weight and declined the heart weight.Kudzu vine extract significantly(P<0.001)suppressed the level of CK-MB,LDH,CK,cTnT and BNR Kudzu vine significantly(P<0.001)improved the level of Na~+,Ca~(2+),K~+and CL~-and decreased the level of urea,magnesium,total protein,albumin and globulin.Kudzu vine remarkably altered the lipid and antioxidant parameters.Additionally,Kudzu vine extract suppressed the level of TNF-α,IL-1β,IL-6 and improved the level of TGF-β,IL-10.DOX-induced cardiac dysfunction exhibited the altered relative abundance of different bacteria and Kudzu vine remarkably restore the relative abundance of different bacteria.Therefore,Kudzu vine extract has beneficial effects against DOX-induced cardiac toxicity via alteration of gut microbiota.