Morpometrical and ribosomal DNA sequence analysis of Globodera rostochiensis and Globodera achilleae from Slovenia

摘要

Differences between two populations of the yarrow cyst nematode Globodera achilleae and one population of the potato cyst nematode G. rostochiensis from Slovenia were analysed using morphological characters of cysts and second stage juveniles and molecular data of the internal transcribed spacer region ITS 1, 5.8S and ITS 2 Globodera achilleae and G. rostochiensis were well differentiated using morphometrical method and PCR-RFLP approach. In addition, sequence alignment among G. rostochiensis and G achilleae was performed which resulted in 76 nucleotide differences; therefore, 91 % sequence similarity score in the rDNA fragment was determined. Sequences of G. achilleae were the most similar to the G. artetnisiae sequences published in NCBI database.Sequence alignment comparison of G. achilleae and G. artemisiae resulted in 8 nucleotide difference and a considerable 99 % sequence similarity was estimated.