First record of Longidorus juvenilis and L leptocephalus (Nematoda: Dorylaimida) in Slovenia and their morphometrical and ribosomal DNA sequence analysis

摘要

Longidorus juvenilis and L. leptocephalus are reported from Slovenia for the first time. All developmental stages of these species were recovered from the rhizosphere of Vitis vinifera L. growing in the north-eastern part of Slovenia. Nematodes were identified using morphological characters of females and juveniles. All these stages are described. A bivulval female of L. juvenilis is also described. In addition, sequences of the D2-D3 expansion region of 28S rRNA gene were analysed. Obtained sequences were compared with sequences of the same and closely related species downloaded from the NCBI database. Cluster analysis of sequences confirmed and supported species identifications. The sequences of L. juvenilis and L. leptocephalus found in Sloveniaand sequences of the same species from databank clustered in two separate clades, respectively. Both clades were supported in bootstrap analyses.