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首页> 外文期刊>Russian journal of genetics >Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows
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Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows

机译:GH,Pit-1和β-LG基因多态性对荷斯坦奶牛产奶量的影响

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摘要

Effect of polymorphic variants of growth hormone (GH), β-lactoglobulin (β-LG), and Pit-1 genes on milk yield was analyzed in a Holstein herd. Genotypes of the cows for these genes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Allele frequencies were 0. 884 and 0. 116 for L and V variants of GH, 0. 170 and 0. 830 for A and B variants of Pit-1, and 0. 529 and 0. 471 for A and B variants of β-LG, respectively. GLM procedure of SAS software was used to test the effects of these genes on milk yield. Results indicated significant effects of these genes on milk yield (P < 0. 05). Cows with LL genotype of GH produced more milk than cows with LV genotype (P < 0. 05). Also, for Pit-1 gene, animals with AB genotype produced more milk than BB genotype (P < 0. 05). In the case of β-LG gene, milk yield of animals with AA genotype was more than BB genotype (P < 0. 01). Therefore, it might be concluded that homozygote genotypes of GH (LL) and β-LG (AA) were superior compared to heterozygote genotypes, whereas, the heterozygote genotype of Pit-1 gene (AB) was desirable.
机译:在荷斯坦牛群中分析了生长激素(GH),β-乳球蛋白(β-LG)和Pit-1基因多态性对牛奶产量的影响。通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法确定这些基因的奶牛的基因型。对于GH的L和V变体,等位基因频率为0. 884和0. 116,对于Pit-1的A和B变体为0. 170和0. 830,对于β的A和B变体为0. 529和0. 471。 -LG,分别。使用SAS软件的GLM程序来测试这些基因对产奶量的影响。结果表明这些基因对产奶量有显着影响(P <0. 05)。具有LL基因型的GH奶牛比具有LV基因型的奶牛生产更多的牛奶(P <0. 05)。同样,对于Pit-1基因,具有AB基因型的动物比BB基因型的动物产生更多的牛奶(P <0. 05)。对于β-LG基因,具有AA基因型的动物的产奶量高于BB基因型(P <0. 01)。因此,可以得出结论,GH(LL)和β-LG(AA)的纯合子基因型优于杂合子基因型,而Pit-1基因(AB)的杂合子基因型是理想的。

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