Histomorphometric Analysis of Tissue Responses to Bioactive Glass Implants in Critical Defects in Rat Calvaria

摘要

The aim of this study was to evaluate the osteogenic behavior of two chemically similar bioactive glass products (Biogran?and Perioglas? implanted in critical bone defects in rat calvaria. Thirty-six transfixed bone defects of 8 mm diameter were made surgically in adult male Wistar rats. The animals were distributed equally into three groups: Biogran (Gl), Perioglas (Gil) and without implant material (control; Gill). The morphology and composition of both bioactive glasses were analyzed by scanning electron microscopy and energy-dispersive spectrometry. Tissue specimens were analyzed at the biological time points of 15, 30 and 60 days by optical microscopy and morphometry, demonstrating biocompati-bility for the tested materials with moderate chronic inflammation involving their particles. Bone neoformation resulted only as a reparative reaction to an intentionally produced defect and was limited to the defect's edges. No statistically sig-