Experimental Research on Variation in Serum Calcium, Phosphorus, Sodium, Potassium and the ESR during Bone Transplant

摘要

The aim of this study was to analyze the plasma concentration of chemical elements in blood after bone transplantation, which, correlated with clinical picture, can give us some useful information in orthopaedics and trauma practice. Measurements were performed on multiparametric automatic analyzer EOS Bravo Forte, Hospitex Diagnostics by wet chemistry with reagent kits in the laboratory Hospitex Diagnostics Platform for Interdisciplinary Research USAMVB of Timisoara. For determination of calcium, phosphorus, sodium and potassium the following dosing methods were used: Serum calcium was determined by colorimetric method with o-cresolphthalein without precipitation. Method for determining inorganic phosphorus was UV method based on phospho-molybdate formation using a Diagnostix Hospitex Monoreagent kit. Potassium Method: turbimetric method. Sodium Method colorimetric method. From the laboratory results obtained by us only the variation of plasma calcium and phosphorus values obtained on one hand and those of potassium and sodium on the other hand were interesting, otherwise other values are not significant. By assessment of calcium and phosphorus levels we notice a marked hypocalcemia at 14 days after surgery, which tends to reach normal levels at 21 days compared to phosphate which still shows a trend of decreasing its values and 21 days after surgery. We cannot draw a definitive conclusion on these experimental obsewations but they warrant further research on a larger lot along with their evolution in the clinic.