Studies Regarding the Influence of the Enzyme Immobilization Methods on the Electrodic Surface Upon the Performances of the Glucose Biosensor

摘要

With a view to improve the performances of the enzymatic biosensors used to determine glucose, glucose oxidase was immobilized on the sensing surface of a glass pH-electrode using different techniques: enzyme deposited on cellophane, cross-linked with glutaraldehyde or entrapped in silica gel, using tetrakis(2-hydroxyethyOorthosilicate (THEOS) as gel precursor. The obtained sensors could be used for glucose assay in 0.75·10~4 M - 10~(-2) M concentration range. The biosensors performances indicate that the sol-gel immobilization technique, using THEOS as precursor, is a good method for enzyme immobilization on the sensors membranes, leading, especially, to the enhancement of their sensitivity.