Iodine-125 Chitosan-Vitamin C Complex: preparation, characterization and application

摘要

In heterogeneous conditions, water soluble Chitosan-Vitamin C Complex (CSVC) is successfully synthesized via the ionic interaction between gamma-degraded CS and VC. Chitosan (CS) of low molecular weight (MW) is prepared using gamma-irradiation method. The coupling of CS and vitamin C (VC) is carried out by the chemical treatment of VC with the gamma-degraded CS. The formation of CSVC complex instead of physical mixture is confirmed by FT-IR and UV spectrometry. Characterization by transmission electron microscope (TEM) and dynamic light scattering (DLS) shows the formation of a nanostructure in 40 nm range. The preparation of labeled CSVC was performed using chloramines-T oxidation method. The labeling feasibility of CSVC nanostructure by Iodine-125 (I-125) is investigated. The optimized conditions of labeling are thought to be 50 mu g of oxidizing agent, pH 3, and one minute reaction time. The Biodistribution activity of I-125 radiolabeled CSVC nanostructure (I-125-CSVC) is examined on a group of different ascites tumor bearing mice. Calculation of the biodistribution percentages shows that the tumor, liver, and kidney are the targeting organs of I-125-CSVC nano-structure.