Evaluation of regeneration potential of sweet sorghum genotypes using immature inflorescences as explants.

摘要

Sweet sorghum is one of the most potentially valuable sources for biofuel production. However, molecular genetic research on sweet sorghum is limited by the lack of suitable genotypes for genetic transformation. Sixty-one diverse genotypes of sweet sorghum were evaluated and determined their callus induction and plant regeneration capabilities using immature inflorescences as explants. Callus was induced from 53 genotypes of sweet sorghum on four types of culture media. The frequency of callus induction exceeded 85% and even reached 100% in six genotypes. The highest callus induction rate was obtained on Y3 medium [MS medium+3 mg/l 2, 4-dichlorophenoxy acetic acid (2, 4-D) +0.5 mg/l kinetin (KT)] with an average induction rate of 43.5%. The amount of phenolic compounds produced varied widely among the different genotypes. Of the 61 genotypes, 32 were able to regenerate into whole plants. The highest frequency of plant regeneration was 87.5%. The sweet sorghum genotypes MN-3013, SILVER TOP-1, FETERITA NIDIANA, POTCH 1002 and MN-81 showed the best callus induction and regeneration capabilities in these conditions. This research has identified the sweet sorghum genotypes with the best potential for callus production and whole-plant regeneration in culture. This information is essential for successful genetic transformation of sweet sorghum.