Cytotoxicity of Some Edible Plants toward Ehrlich Ascites Carcinoma Cells

摘要

The goal of this study was to perform some contribution into the knowledge on the antitumor ability of plants, traditionally used in folk medicine in Armenia. The water and ethanol extracts of dried leaves of grape, sorrel and sea buckthorn, rose petals, melilot, pellicles of walnut kernels were prepared. Their ability to suppress the growth of cultivated mice Ehrlich Ascites Carcinoma (EAC) cells and Mice Peripheral Blood Leukocytes (MBL) was screened. The ethanol extracts of rose petals, grape and sorrel leaves were fractionated by gel-filtration. The extracts and obtained fractions were characterized by antioxidant activity, qualitative chemical analysis, thin layer chromatography and UV-Vis absorbance. The IC_(50) values for inhibition of EAC cells' growth by ethanol extracts of the three plants were evaluated and appeared in nanogram range. IC_(50) values for DPPH and ABTS radicals' scavenging by ethanol extracts were evaluated. The antioxidant activities of the three studied extracts of plants were in the order: rose petals>grape leaves>sorrel leaves. The most of phenol glycosides from the three plants with strong cytotoxicity toward EAC cells and practically no effect on the MBLs, possessed relatively lower antioxidant activity. The strong toxicity of the extract of sorrel leaves as a whole and of its phenol glycoside and anthracene derivative fractions to EAC cells and low action on MBLs underlined the preference of sorrel leaves as an anticancer remedy. Based on the obtained results, it can be assumed that the extracts of the studied plants and their constituents may be recommended as sources for anticancer drugs.