Lactobacillus pentosus var. plantarum C29 ameliorates age-dependent memory impairment in Fischer 344 rats

摘要

To understand the anti-inflammaging effect of lactic acid bacteria, we selected NF-B activation-inhibitory Lactobacillus pentosus var. plantarum C29 and investigated its memory-enhancing and anti-inflammatory effects in aged Fischer 344 rats. C29 (2x10(9)CFUrat(-1)), which was orally administered once a day (6days per week) for 8weeks, significantly restored age-reduced spontaneous alternation to 952% of that seen in young rats (P<005). C29 treatment also shortened the escape latency on the 4th day to 538% of that seen in young rats (P<005). Twenty hours after the last training session, C29 significantly increased the swimming time within the platform quadrant, which was shortened in the aged control rats. Oral administration of C29 restored age-reduced doublecortin (DCX) and brain-derived neurotrophic factor (BDNF) expression and cAMP response element binding protein (CREB) activation in aged rats. Treatment of aged rats with C29 suppressed the expression of p16, cyclooxygenase-2, and inducible nitric oxide synthase, as well as the activation of Akt, mTOR, and NF-B in the hippocampus. These findings suggest that C29 ameliorates ageing-dependent memory impairment by inhibiting NF-B signalling pathway, inducing DCX and BDNF expression and activating CREB.