Regeneration of plants from cell suspension cultures and encapsulated cell suspension cultures of Solanum tuberosum L. cv. Clarissa

摘要

Callus cultures of the cultivar Clarissa were initiated from the end pieces of internode explants producing somatic embryos. The chopped somatic embryos and the basis produced soft callus when cultured on solid Murashige and Skoog media containing 1 mg/L 2,4-dichlorophenoxyacetic acid. Cell suspension cultures were established from these calli. They contained small cell clusters and single large vacuolated cells of variable shape. The embryogenic potential was tested on solid media. Four cell lines could be selected which maintained their embryogenic potential over a long period of time. Regenerates transferred to the greenhouse produced plants and tubers not distinguishable from the control plants.