Secretion of heterologous proteins of therapeutical interest in Lactococcus lactis [French]

摘要

The GRAS (for Generally Regarded As Safe) bacterium, Lactococcus lactis, is a good candidate to produce heterologous proteins of vaccinal, medical or technological interest. The secretion of such proteins would allow a direct contact between the protein and its target and therefore would be a better mode of production than intracellular production. To develop heterologous protein export systems in L. lactis, we used the staphylococcal nuclease. We combined these secretion tools to the Nisin-Inducible Controlled Expression system. We first developed two new expression vectors allowing the inducible production of Nuc intra- and extracellularly. We then used them to produce in L. lactis two heterologous proteins of therapeutical interest: (i) the ovine omega interferon which possesses antiviral properties and which will be useful to enhance immune response in the case of the use of L. lactis as antigen delivery vehicle and (ii) a fusion protein between the Blg41-60 epitope and the mature part of Nuc.