Ultra-Fast LC–MS-MS Analysis of 25-0H Vitamin D2 and D3 from Serum

Philip J. Koerner

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Ultra-Fast LC–MS-MS Analysis of 25-0H Vitamin D2 and D3 from Serum

机译：血清中25-0H维生素D2和D3的超快速LC-MS-MS分析

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摘要

An analytical method for the rapid determination of 25-OH vitamin D2 and 25-OH vitamin D3 in serum, which will speed diagnosis of potential Vitamin D deficiencies, is described. The method utilizes protein precipitation and ultra-fast LC-MS-MS using a Kinetex 2.6 pm core-shell column.

机译：描述了一种快速测定血清中25-OH维生素D2和25-OH维生素D3的分析方法，该方法可加快潜在维生素D缺乏症的诊断。该方法利用Kinetex 2.6 pm核壳色谱柱进行蛋白质沉淀和超快速LC-MS-MS。

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《LC GC North America》 |2011年第s1期|共1页
作者
Philip J. Koerner;
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正文语种 eng
中图分类分析化学;
关键词
Analysis; Serum; core-shell column;

机译：分析血清核壳柱;

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1. Ultra-Fast LC–MS-MS Analysis of 25-0H Vitamin D2 and D3 from Serum [J] . Philip J. Koerner LC GC North America . 2011,第S1期

机译：血清中25-0H维生素D2和D3的超快速LC-MS-MS分析
2. Analytical measurement of serum 25-OH-vitamin D3, 25-OH-vitamin D2 and their C3-epimers by LC-MS/MS in infant and pediatric specimens [J] . YazdanpanahM., BaileyD., WalshW., Clinical Biochemistry . 2013,第13a14期

机译：LC-MS / MS法测定婴幼儿标本中血清25-OH-维生素D3、25-OH-维生素D2及其C3-受体的分析方法
3. Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC-MS/MS methods not separating both metabolites as determined by an LC-MS/MS method for separate quantification of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum [J] . Johannes M.W. van den Ouweland, Antonius M. Beijers, Henny van Daal Journal of chromatography, B. Analytical technologies in the biomedical and life sciences . 2014,第Null期

机译：在LC-MS / MS方法中不能通过分离3-Epi-25-羟基维生素D3的电离效率提高对25-羟基维生素D3的高估，如通过LC-MS / MS方法对25-羟基维生素D3、3进行单独定量所确定的那样人血清中的-epi-25-羟基维生素D3和25-羟基维生素D2
4. Total Solution for the Analysis of 25-Hydroxy Vitamin D2 and D3 in Human Serum Using Automated Sample Preparation and UHPLC-TOF [C] . Alexander Wahba, Sean Sales, Sean Daugherty American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：使用自动样品制备和UHPLC-TOF分析人血清中25-羟基维生素D2和D3的总溶液
5. The effect of vitamin D2, vitamin D3 or vitamin D2 in mushroom powder supplements on broad gene expression in human white blood cells. [D] . Feigert, Caroline Elizabeth. 2014

机译：蘑菇粉中的维生素D2，维生素D3或维生素D2对人白细胞中广泛基因表达的影响。
6. Development and optimization of an LC-MS/MS-based method for simultaneous quantification of vitamin D2 vitamin D3 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 [O] . Jiri Adamec, Amber Jannasch, Jianjie Huang, -1

机译：发展和维生素D2维生素D325-羟基D2和25-羟基维生素D3同时定量的基于ms-LC-ms /方法的优化
7. Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: a systematic review and meta-analysis. [O] . Tripkovic, L, Lambert, H, Hart, K, 2012

机译：比较维生素D2和补充维生素D3在提高血清25-羟基维生素D状态中的作用：系统评价和荟萃分析。

Ultra-Fast LC–MS-MS Analysis of 25-0H Vitamin D2 and D3 from Serum

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