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首页> 外文期刊>FEBS letters. >Solvent isotope and viscosity effects on the steady-state kinetics of the flavoprotein nitroalkane oxidase
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Solvent isotope and viscosity effects on the steady-state kinetics of the flavoprotein nitroalkane oxidase

机译:溶剂同位素和粘度对黄酮蛋白硝基烷氧化酶稳态动力学的影响

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摘要

The flavoprotein nitroalkane oxidase catalyzes the oxidative denitrification of a broad range of primary and secondary nitroalkanes to yield the respective aldehydes or ketones, hydrogen peroxide and nitrite. With nitroethane as substrate the D2O(kcat/KM) value is 0.6 and the D2Okcat value is 2.4. The kcat proton inventory is consistent with a single exchangeable proton in flight, while the kcat/KM is consistent with either a single proton in flight in the transition state or a medium effect. Increasing the solvent viscosity did not affect the kcat or kcat/K M value significantly, establishing that nitroethane binding is at equilibrium and that product release does not limit kcat.
机译:黄酮蛋白硝基链烷氧化酶催化多种伯和仲硝基链烷的氧化反硝化反应,生成相应的醛或酮,过氧化氢和亚硝酸盐。以硝基乙烷为底物,D2O(kcat / KM)值为0.6,而D2Okcat值为2.4。 kcat质子库存与飞行中的单个可交换质子一致,而kcat / KM与过渡状态或中等效应在飞行中的单个质子一致。增加溶剂粘度不会显着影响kcat或kcat / K M值,这表明硝基乙烷键合处于平衡状态,并且产物释放不会限制kcat。

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