REACTION OF CAPSICUM GENOTYPES TO OBUDA PEPPER VIRUS, TOBACCO MOSAIC VIRUS AND CUCUMBER MOSAIC VIRUS

摘要

In 2003 year, research on virus susceptibility and resistance of Capsicum genotypes has been continued. The objective of this year in this respect was to examine the reaction of 29 Capsicum genotypes to common strain of Tobacco mosaic virus (TMV-C/U1), Obuda pepper virus (ObPV), (syn.: Ob strain of Tomato mosaic virus, ToMV-Ob) and legume strain of Cucumber mosaic virus (CMV-U/246) Mechanically inoculated plants were symptomatologically tested for infections, Five weeks after inoculation the infectedplants were tested by direct double-antibody sandwich ELISA (DAS ELISA) method. Test samples were considered susceptible to viruses if their extinction values exceeded three times than those of the healthy control ones. In order to confirm the results of symptomatology and serology, back inoculations were also carried out to Nicotiana glutinosa, N. tabacum 'Xanthi-nc' and N. tabacum 'Samsun'. Among the tested Capsicum genotypes one (VI-13-13/96 ii) to TMV-C/U1, and one [VI-91 (179) 47/87] to ObPV showed resistance, Neither local nor systemic symptoms on inoculated plants could be observed and results of DAS ELISA and back inoculation were also negative. Six genotypes [XII-a=542, XII-a=543, XII-a=407 French Perennial, XII-a=9/99 F_1 x (RR DH x 412/a CMVR), XII-a=15/99 F_1 x (413 x R12 DH x R KDH) BC_1 F_1, XII-a-4/99 F_2] were resistant to CMV-U/246. These genotypes could be used for resistance breeding to viruses.