Simultaneous estimation of hydroxychavicol and chlorogenic acid from Piper betel L. through RP-HPLC

摘要

A RP-HPLC method was developed ( max=280) to quantify hydroxychavicol and chlorogenic acid in Piper betel Linn. The method was validated for linearity, limit of detection (LOD=3:1/S), limit of quantification (LOQ=10:1/S), precision, accuracy and ruggedness. The response was linear with good correlation between concentration and mean peak area through a coefficient of determinants (r 2) of 0.9940, y=1.98e+004x+5.19e+004 and 0.9945, y=2.76e+004x+1. 40e+005 with LOD 1.6gmL 1, 1.0gmL 1 and LOQ 5.0gmL 1 and 3.0gmL 1, respectively, for hydroxychavicol (28.56% w/w) and chlorogenic acid (0.40% w/w). The %RSD of precision and recovery of hydroxychavicol and chlorogenic acid were 2.0%. The proposed method was simple, accurate, specific, precise and reproducible.