反相高效液相色谱法测定对羟基肉桂酸口服液中对羟基肉桂酸的含量

摘要

本试验旨在建立高效液相色谱法测定对羟基肉桂酸口服液中对羟基肉桂酸的含量.采用Waters XTerra RP18(4.6 mm×150mm,5μm)色谱柱,以甲醇-1%乙酸溶液(40:60)为流动相,检测波长为310nm,流速1mL/min,温度为室温.结果显示对羟基肉桂酸在2-500μg/mL范围内,线性关系良好(r=0.999952),相对标准偏差(RSD)为0.5%,平均回收率为98.22%-101.07%.该方法简便、快速、准确,可用于对羟基肉桂酸口服液的质量控制.%It was aimed to establish high performance liquid chromatography( HPLC) method for determination of p-coumarie acid (PCA) in PCA solution.The p-coumaric acid was successfully separation performed on XTerra(R) RP18 column at ambient temperature, MeOH-1 ％ acetic acid (40 : 60) was the mobile phase, the detection wavelength was 310 nm and flow-rate 1 mL/min.The results showed that there was good liner relationship(r=0.999904) in the range of 2 to 500 μg/mL,RSD=0.5％, the average recovery rate was 98.22％ to 101.07％.The results showed that the simple, rapid and accurate method could be used for the quality control of PCA solution.