1argeted optogenetic stimulation and recording If neurons in vivo using cell-type-specific xpression of Channelrhodopsin-2

摘要

major long-term goal of systems neuroscience is to identify the different rotes of neural subtypes in brain circuit function. Theility to causally manipulate selective cell types is critical to meeting this goal. This protocol describes techniques for opticallymutating specific populations of excitatory neurons and inhibitory interneurons in vivo in combination with electrophysiology.tt type selectivity is obtained using Cre-dependent expression of the light-activated channel Channelrhodopsin-2. We also scribe approaches for minimizing optical interference with simultaneous extracellular and intracellular recording. Thesetogenetic techniques provide a spatially and temporally precise means of studying neural activity in the intact brain and ow a detailed examination of the effect of evoked activity on the surrounding local neural network. Injection of viral vectorswires 30-45 min, and in vivo electrophysiology with optogenetic stimulation requires 1-4 h.