Drift time-specific collision energies enable deep-coverage data-independent acquisition proteomics

摘要

We present a data-independent acquisition mass spectrometry method, ultradefinition (UD) MS~E. This approach utilizes ion mobility drift time-specific collision-energy profiles to enhance precursor fragmentation efficiency over current MS~E and high-definition (HD) MS~E data-independent acquisition techniques. UDMS~E provided high reproducibility and substantially improved proteome coverage of the HeLa cell proteome compared to previous implementations of MS~E, and it also outperformed a state-of-the-art data-dependent acquisition workflow. Additionally, we report a software tool, ISOQuant, for processing label-free quantitative UDMS~E data.