Determination of Meat Authenticity using Comprehensive Proteomic Strategy, Data-Independent Acquisition and High-Resolution Mass Spectrometry

摘要

The application of a bottom-up proteomic strategy applied to meat speciation was tested using targeted tryptic peptide biomarkers. As shown, myoglobin proteotypic peptides can be used to preform meat speciation. Moreover, thorough in silico protein sequence analysis and tryptic digestion allowed the identification of three other proteotypic peptide biomarkers (i.e. Myosin-1 619-638, Myosin-2 619-639 and β-Haemoglobin peptide 40-58). Results obtained were analogous and allow to strengthen results conclusion. Likewise, other species-specific peptides can be used including Tropomyosin and myosin light chain 1/3 related biomarkers. Targeted MS~2 or DIA methods can be successively used with different purposes. Targeted MS~2 methods can be fully validated for meat authentication and adulteration analysis. Moreover, labeled internal standards can be synthesized and used for quality control purposes in routine analysis laboratories. For research, DIA provides a more detail dataset and allow comprehensive data mining.