Isolation and Optimal Culture Conditions of Prodigiosin-like Pigment Produced by Zooshikella sp JE-34.

摘要

Isolation and Optimal Culture Conditions of Prodigiosin-like Pigment Produced by Zooshikella sp. JE-34. Kim, Ju-Sang, Man-Chul Kim, Kyeong-Jun Lee(1), and Moon-Soo, Heo*. Department of Aquatic Life Medicine & Marine and Environmental research Institute, Jeju National University, Jeju 690-756, Korea, (1)Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea - A bacterial strain, JE-34, producing a high concentration of red pigment was isolated from a sediment in East China Sea. It was identified as Zooshikella sp. JE-34 based on the 16S rRNA gene sequence analysis. The red pigment was purified by solvent extraction and HPLC was identified as prodigiosin-like compound. Nutritional and cultural conditions were optimized for the production of prodigiosin-like pigment in the flask level. Optimal Culture conditions were at initial medium pH 6.0 similar to 7.0, 30 degrees C and 4 days incubation. For carbon and, nitrogen sources were soluble starch and malt extract.