1株甲基杆菌Methylobacterium sp.WGM16的鉴定及降解甲醇的最佳培养条件

摘要

从水稻根部土壤中筛选到1株粉红色、需氧的兼性甲基营养型菌株WGM16,该菌为革兰阴性杆菌.根据菌株16S rRNA基因序列比对分析及结合菌株常规形态特征、生理生化性状的鉴定,将该菌初步鉴定为Methylobacterium sp.PCR扩增到菌株WGM16编码甲醇脱氢酶a-亚基的mxaF基因,表明菌株WGM16中存在甲基营养代谢途径.在培养温度为32℃、以1%的甲醇作为碳源、pH值为8.0的培养条件下,其甲醇降解率可这75%.%A pink, aerobic, and facultative methylotrophic bacterium strain WGM16, was isolated and screened from rice's root soil. It was Gram-negative rod. Comparison analysis of 16S rRNA gene sequence and physiological and biochemical characteristics combined with routine morphological, physiological and biochemical characteristics identification, the strain WGM16 was identified as a member of the genus Methylobacterium sp. PCR amplification showed the gene coding for the α-sub-radical of methanol dehydrogenase (mxaF) present in the strain WGM16, indicating there existed a methylotrophic metabolism. Under the conditions of temperature at 32 ℃ , with 1％ of methanol as carbon source, pH 8.0, the methanol degradation rate was as high as 75％.