Profiles of toxin genes and antibiotic susceptibility of Bacillus cereus isolated from perilla leaf and cultivation areas.

摘要

Two hundred Bacillus cereus isolates from perilla leaf cultivation areas in Miryang, Korea were investigated for toxin producing genes and antibiotic susceptibility. The isolates were identified to belong to 11 toxigenic groups by toxin gene profiles. 21% of strains isolated from the perilla leaves had both enterotoxin and emetic toxins. Toxin genes entFM (100%), nheA (100%) and hblA, C, D (65.5%) were frequently found in the perilla leaves, whereas EM (21.0%) was less common. Most isolates were susceptible to 10 antibiotics, but they were highly resistant to penicillin (100%), ampicillin (100%), oxacillin (94.9%), amoxicillin-clavulanic acid (95.6%), cefazolin (78.2%), and rifampicin (58.0%). These results indicate that B. cereus from perilla leaves cultivated in the Miryang area of Korea might lead to foodborne outbreaks of diarrhoea and vomiting.