Cirsium japonicum Extracts Show Antioxidant Activity and PC 12 Cell Protection against Oxidative Stress

摘要

The phenolic compounds, antioxidant activity and neuronal cell protective effect of Cirsium japonicum extract were evaluated in this study. High performance liquid chromatography mass analysis showed that C. japonicum was composed of chlorogenic acid,linarin, and pectolinarin. C. japonicum extract showed its antioxidant activity with half-maximal inhibitory concentrations of 567 and 130ug/mL by DPPH and ABTS radical scavenging activity, respectively. The total antioxidant capacities of C. japonicumvia DPPH, ABTS, and FRAP assays were 11.32, 100.15, and 12.76 ug/ mL trolox equivalents, respectively. In addition, the neuroprotective effect of C. japonicum extract was investigated by measuring cell viability via MTT, LDH and DCF-DA assay using H202-damaged PC 12 cells. C. japonicum extract showed neuronal cell protective effects in a dose-dependent manner. These results indicated that C. japonicum extract has potent antioxidant and neuronal protective effects. Therefore, C. japonicum can be regardedas an effective and safe functional food resource as natural antioxidants, and may decrease the risk of neurodegenerative disorders.