Isolation and identification of growth inhibtion substance on L. monocytogenes from Dystaenia takesimana Kitagawa

摘要

The ethanol extracts and its n-hexane fraction of Dystaenia takesimana Kitagawa exhibited growth inhibition on Listeria monocytogenes ATCC 19111, ATCC 19112, ATCC 19113, ATCC 19114 and ATCC 15313. The minimum inhibitory concentration of the ethanol extract and its n-hexane fraction were 50 ppm and below 30 ppm on Listeria monocytogenes respectively. By silica gel column chromatography, the active fraction A8 was obtained from the ethanol extract of Dystaenia takesimana Kitagawa. After three times of column chromatography, the SBD-1 and SBD-2 were separated from the A8 fraction of the ethanol extract of Dystaenia takesimana Kitagawa. Antimicrobial activity of the SBD-1 and SBD-2 was lower than that of the A8. And the A8 exhibited growth inhibition on five strains of Listeria monocytogenes at the level of 10 approx 30 ppm and the bactericidal effect was confirmed at same the level . The purified antimicrobiai active compound was identified as (9z)-heptadeca-1,9-dien-4,6-diyn-3,8-diol, falcarindiol, by EI/MS, ~1H-NMR and ~(13)C-NMR.