Neuronal cell protective effects of methanol extract from cheonggukjang using in vitro system.

摘要

In this study, the neuronal cell protective effects of methanol extract of cheonggukjang were evaluated. Proximate composition and total phenols content of the methanol extract were 40.95% crude protein, 22.49% crude fat, 15.99% nitrogen free extract, 7.91% moisture, 6.74% crude ash, 5.92% crude fibre, and 28.43 mg/g of total phenols. Intracellular reactive oxygen species accumulation resulting from H₂O₂ treatment of PC12 cells was significantly reduced when the methanol extract of cheonggukjang was present in the media compared to PC 12 cells treated with H₂O₂ without the methanol extract. In a cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT), the methanol extract showed protective effects against H₂O₂ -induced neurotoxicity; lactate dehydrogenase release into the medium was also inhibited. The methanol extract of cheonggukjang also showed inhibitory activity against acetylcholinesterase, which was dose dependent.