Production condition, purification and properties of anticaries microbial agent by Bacilluse alkalophilshaggy JY-827

摘要

The study was performed to investgate the excellent microbial anticaries substance which is more effective than the chlorhexidine in the dental caries treatment. A typical strain which produced the most excellent antimicrobial substance was selected, and identified novel alkalophillic Bacillus alkalophilshaggy JY-827. For the maximal production of the microbial antibiotic against Streptococcus mutans from B. alkalopilshaggy JY-827, the optimal culture condition was in the medium containing glucose 15 g/L, pepton 10 g/L and K_2HPO_4 2 g/L, the highest production of antibiotic against S. mutans was obtained at 25 deg C and pH 11.0 for 5 days. The antibiotic from B. alkalophilshaggy JY-827 was purified by organic solvent extraction, silica gel and sephadex LH-20 column chromatograpies, and then crystallized with methanol. The crystallin compomation of this antibiotic was as a curcular shape. The melting point and rm[#alpha#]_D~(20) were 152-154 deg C and +55 deg, respectively. Based on Instumental analyses such as FT-IR, ~1H-NMR, ~(13)C-NMR and GC-mass, the antibiotic was identified as aminoglycoside. It was obtained as amorphous white powder, and soluble in water, methanol but insoluble in ether, chroloform. This antibiotic inhibited the growth of S. mutans to about 3 day at the concentration of 2.5 X 10~(-7) M. It was stable at the alkaili condition but unstable within the acid condition. It was also stable up to 70 deg C.