Molecular Identification of the Dominant Species of Dark-winged Fungus Gnat (Diptera: Sciaridae) from Button Mushroom (Agaricus bisporus) in Korea

摘要

The dark-winged fungus gnats are one of the most serious fly pests attacking the mushroom cultivation in Korea. They cause severe damage to the artificial sawdust beds used to cultivate mushroom, and reduce the production of button mushroom, Agarkus bisporus, in greenhouses. In this study, we collected nine species of the mushroom flies in order to identify the dominant species of the dark-winged fungus gnat attacking the A. bisporus plantation using the yellow sticky trap in Buyeo-gun, Boryeong-gun,Yongin-si and Chilgok-gun from April to June 2015. The collected samples were used to determine the DNA sequence of the cytochrome c oxidase subunit I (COI) of the nine different species by DNA barcoding. The sequencing results showed that Lycoriella ingenua was the dorninanl dark-winged fungus gnat species destroying A. bisporus cultivated on the artificial sawdust beds in Korea.