APPLICATION OF BIOLUMINESCENCE METHOD FOR QUALITY CONTROL OF CULTURE MEDIA AND BACTERIA APPLIED TO STANDARD NORM (NMX-AA-042-1987)

摘要

The aim of the study was to develop a bioluminescence assay for the rapid monitoring of the quality of the bacterial culture medium and quality of standard bacterial strain by way of ATP detection,as well as to define the relationship between ATP and the bacterial population estimated by MPN and CFU methods.The growth kinetics of E.coli in the brilliant green bile and lactose broth,suggested by the NOM (Mexican Official Norms) were studied.The influence of pH,ionic strength,and temperature was also evaluated for E.coli and E.coli co-inoculated with Pseudomonas spp.ATP was detected by the bioluminescent method after dimethyl sulfoxide treatment and simultaneously the bacteria present in the samples were enumerated by plating and MPN techniques.All the parameters tested to compare the growth of bacteria in brilliant green bile and lactose broth were statistically significant.A strong inhibition of growth was observed in both media containing 5% NaCl.An increase in the specific growth constant of E.coli was noted in the presence of Pseudomonas spp.The relationship between ATP,CFU,and MPN of the cultures was established and it was observed that the ATP assay has good correlation with CFU and MPN techniques.