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Determination and analysis of the complete mitochondrial genome sequence of Taoyuan chicken

机译:桃园鸡线粒体全基因组序列的测定与分析

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摘要

Taoyuan chicken is excellent native breeds in China. This study firstly determined the complete mitochondrial genome sequence of Taoyuan chicken using PCR-based amplification and Sanger sequencing. The characteristic of the entire mitochondrial genomewas analyzed in detail, with the base composition of 30.26% A, 23.79% T, 32.44% C, 13.50% G in the Taoyuan chicken (16,784 bp in length). It contained 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and a major non-coding control region (D-loop region). The complete mitochondrial genome sequence of Taoyuan chicken will be useful for the phylogenetics of poultry, and be available as basic data for the genetics and breeding.
机译:桃园鸡是中国优良的本土品种。本研究首先利用基于PCR的扩增和Sanger测序技术确定了桃园鸡的线粒体基因组完整序列。详细分析了整个线粒体基因组的特征,在桃园鸡(长16,784 bp)中碱基组成为30.26%A,23.79%T,32.44%C,13.50%G。它包含2个核糖体RNA基因,13个蛋白质编码基因,22个转移RNA基因和一个主要的非编码控制区(D-loop区)。桃园鸡线粒体基因组的完整序列,对于家禽的系统发育将是有用的,并可作为遗传学和育种的基础数据。

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