Investigation of Biochemical Basis for Differentiating Necrosis from Apoptosis in Leukemic Cells using Raman Spectroscopy

摘要

Apoptosis and necrosis are the two distinct modes of cell death in the human body [1]. The disturbance of the fine balance between apoptosis and necrosis may be responsible for the onset of many diseases [2]. Rapid and accurate identification of apoptosis and necrosis are thus important in the development of therapeutic interventions. Raman spectroscopy has been used to study cell death; however the traditional Raman spectra analysis method is unable to elucidate the changes in the amount of individual basic biochemical components.