Determination of Nucleic Acid at Nanogram Levels with Manganese-Tetrasulfonatophthalocyanine Sensitized by Cetyltrimethylammonium Bromide Using a Resonance Light-Scattering Technique

摘要

This is the first report on the determination of deoxyribonucleic acids based on the enhancement of resonance light-scattering (RLS) of manganese-tetrasulfonatophthalocyanine (MnTSPc) by DNA and cetyltrimethylammonium bromide (CTMAB). Under the conditions of pH 10.60～11.85 and an ionic strength of 0.01 mol L~(–1) (NaCl), the interaction of MnTSPc with DNA sensitized by CTMAB results in enhanced RLS signals at 314nm, 346.2nm, 452.6 nm and 494.4 nm in the enhanced regions. It was found that the enhanced RLS intensity at 346.2nm was proportional to the concentration of DNA in the suitable ranges. The limits of detection were 11.3 ng mL~(–1) for fsDNA and 22.9 ng mL~(–1) for ctDNA when the concentration of MnTSPc was chosen to be 2.0 *10~(–6) mol L~(–1). This method was simple, rapid, and the dye (MnTSPc) was easily synthetized, inexpensive, and stable. We applied it to the determination of DNA in synthetic samples with satisfactory results.