Use of thiopyropyl sepharose for preparation of 2-nitro-5-thiobenzoic acid

摘要

A simple method is described for preparation of 2-nitro-5-thiobenzoic acid(NTB) ,a compound with versatile applications but is commercially unavailable.In this method,NTB is produced by the reduction of 5,5-dithiobis(2-nitrobenzoic acid)(DTNB) with thiopropyl sepharose,which is then separated from NTB by filtration.Two molecules of NTB can be generated from each molecule of DTNB when a large excess of thiopropyl sepharose is used.Thiopropyl sepharose is commercially available and can be readily regenerated for repeated use inthe preparation of NTB.This mehtod is simple and more effective than any othe rreported methods of NTB preparation.