Evaluation of antioxidant status of female diabetic patients in Nnamdi Azikiwe University Teaching Hospital, Anambra State, Nigeria.

摘要

Diabetes mellitus has become an onerous disease to developing countries such as Nigeria. Rapid acceptance of urbanisation and sedentary life styles pose an encumbrance to its prevention and management. Increased oxidative stress in diabetes mellitus has been implicated as a culprit in perpetuating antioxidant depletion and diabetic complications in diabetes mellitus individuals. This study aims to evaluate the level of antioxidant status in type 2 diabetes mellitus (DM) female participants visiting the outpatient diabetic clinic of Nnamdi Azikiwe University Teaching Hospital (NAUTH), Nnewi, Anambra State, Nigeria. A total of 86 participants aged 51 +/- 10 years were recruited for this study. The test group consists of 43 already confirmed type 2 diabetes mellitus females, while the control group consists of 43 apparently healthy females. The test subjects were further subgrouped into good and poor glycaemic control groups, using a cut-off of <7% for HbA1c. Whole blood was collected from participants and aliquoted into specified sample containers for analysis of the following parameters: random blood glucose (RBG; mg/dL), glycosylated haemoglobin (HbA1c; %), glutathione reductase (GR; U/L) and total antioxidant status (TAS; mmol/L). Results from this study showed that the mean differences in RBG (197.74 +/- 49.29 mg/dL) and HbA1c (9.86 +/- 1.44%) were significantly higher in the test group compared to the control group RBG (104.79 +/- 11.33 mg/dL) and HbA1c (5.21 +/- 1.23%) (P < 0.05). The mean differences of GR (45.81 +/- 20.45 U/L) and TAS (1.81 +/- 1.04 mmol/L) were significantly lower in the test group compared to the control group GR (61.21 +/- 14.34 U/L) and TAS (2.73 +/- 2.08 mmol/L) (P < 0.05). The poor glycaemic test group was observed to have the highest RBG (200.34 +/- 50.4 mg/dL) and HbA1c (10.23 +/- 1.33%) compared both to good glycaemic test group RBG (186.38 +/- 45.39 mg/dL), HbA1c (6.54 +/- 0.45%) and non-diabetic group RBG (104.79 +/- 11.33 mg/dL) and HbA1c (5.21 +/- 1.23%) (P<0.05). Glutathione reductase (40.66 +/- 15.48 U/L) and TAS (1.80 +/- 1.08 mmol/L) were significantly more depleted in the poor glycaemic test group compared to the non-diabetic group GR (61.21 +/- 14.34 U/L), TAS (2.73 +/- 2.08 mmol/L) and good glycaemic test group GR (68.38 +/- 25.09 U/L), TAS (1.86 +/- 0.92 mmoVL) (P<0.05). Out of the 43 participants in the test group, only 18.6% had good glycaemic control and 81.4% had poor glycaemic control. There were significant negative correlations between RBG and TAS (r=-0.260; P=0.015); RBG and GR (r=-0.403; P=0.000) and HbA1c and GR (r=-0.471; P=0.000) (P<0.05). However, HbA1c and TAS showed no significant correlation (r=-0.170; P=0.119) (P>0.05). This study concludes that there is antioxidant depletion in females with type 2 diabetes.