Linkage mapping of the tumor necrosis factor receptor 2 (TNFR2) gene to 1p36.2 using the single-strand conformation polymorphism technique

摘要

We identified two informative polymorphisms in the transcribed 3′ untranslated region of the tumor necrosis factor receptor 2 (TNFR2) gene using the poly-merase chain reaction (PCR) with the single-strand conformation polymorphism (SSCP) technique. These poly-morphisms demonstrated Mendelian inheritance and were useful for linkage analysis. TNFR2 was very closely linked to the pronatriodilatin gene (PND), and theTNFR2SSCP polymorphisms were much more informative than the restriction fragment length polymorphisms currently available for thePNDlocus. In addition, we have demonstrated that genotyping could be performed with DNA obtained from paraffin-embedded tissu